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Nosocomial Infections in ICU · Journal article
Antibiot Khimioter = Antibiotics and Chemotherapy · September 5, 2026
Early or partial results. Treat as a signal, not a conclusion.
This is a descriptive molecular characterization of 35 clinical Klebsiella pneumoniae isolates from three Russian cities, identifying their sequence types, antibiotic resistance genes, virulence genes, and efflux pump profiles. The work documents the prevalence of successful international clones and multi-drug resistance determinants in a regional collection but does not test clinical efficacy, compare outcomes, or provide quantitative evidence for practice recommendations.
Descriptive retrospective strain characterization. Clinical K. pneumoniae strains isolated from lower respiratory tract, blood, and urine specimens from patients at facilities in Moscow, Vologda, and Yakutsk during 2021–2023. Included neurosurgical and COVID-19 patients.. n = 35. Moscow, Vologda, and Yakutsk, Russia; strains deposited in State Clinical Hospital 'Obolensk' collection..
ST512 (KL37/107) and ST395 (KL2/39) genetic lineages were predominant among the 35 strains Resistance genes identified included bla SHV, bla TEM-1B, bla CTX-M-15, bla KPC-3, bla NDM-1, and bla OXA-48 across the collection Virulence genes wabG, fimH, and uge_2 were detected in all 35 strains
No clinical outcomes (mortality, treatment response, length of stay) reported; purely genomic characterization.
Clinicians and infection prevention specialists should recognize that K. pneumoniae in this Russian regional cohort is dominated by established high-risk clones carrying multiple carbapenem and aminoglycoside resistance genes. This supports surveillance and empiric therapy considerations in similar settings but does not directly guide individual patient management without clinical correlation.
Descriptive genomic and molecular characterization of 35 clinical strains with no comparator, control group, or clinical outcome measure; establishes baseline resistance and virulence profiles but does not test an intervention or hypothesis with inferential statistics.
As stated by the source record.
Quoted from the source exactly as published.
Clinicians and infection prevention specialists should recognize that K. pneumoniae in this Russian regional cohort is dominated by established high-risk clones carrying multiple carbapenem and aminoglycoside resistance genes. This supports surveillance and empiric therapy considerations in similar settings but does not directly guide individual patient management without clinical correlation.
Graded across the dimensions that decide whether you should act, each from what the source actually supports. There is no single score, and where a dimension was not assessed it says so.
Background. Klebsiella pneumoniae is a clinically significant pathogen associated with a broad spectrum of infectious diseases, characterized by genetic diversity and the formation of successful clones. The aim of the study was to analyze the molecular genetic characteristics of clinical K. pneumoniae strains ( n = 35) isolated from the lower respiratory tract, blood, and urine of patients in Moscow, Vologda, and Yakutsk in 2021–2023 and deposited in the collection of the State Clinical Hospital «Obolensk». Material and methods. Bacteria were identified using a MALDI-TOF Biotyper (Bruker, Germany), and antimicrobial susceptibility was determined using a Vitek 2 Compact (BioMireux, France) and serial dilution in broth. Antibiotic resistance and virulence genes ( rmpA, iroN, iroD, uge, wabG, kfu, fimH, allS, and allR ) were detected via PCR. Whole genome analysis (GenoLab M) was performed using BLAST, PubMLST, and other methods. Results. Strains were isolated from neurosurgical and COVID-19 patients in Moscow. The ST512 (KL37/107) and ST395 (KL2/39) genetic lineages were predominant, with ST874 (KL45), ST147 (KL64), ST336 (KL25), ST11 (KL27), and ST1773 (KL123) being less common. The following antibiotic resistance genes were identified: bla SHV-106/121/28/182, bla TEM-1B, bla CTX-M-15, bla KPC-3, bla NDM-1, bla OXA-48, armA, aph (3›)-VI; aac (3)-IIa/d, aac (6›)-Ib-cr; aph (3›)-Ia/VI, aadA2, sul1/2, fosA, catA1/B3, msrE, mphA, ermB, tetA. The strains isolated in Vologda from patients with COVID-19 complicated by pneumonia were classified as ST874(K45), ST395(K64), and carried the genes bla NDM-1/5; bla TEM-1B, bla SHV-121, bla CTX-M-15, armA, aph (3›)-VI; aac (3)-IId, aac (6›)-Ib-cr; aadA2, sul1/2; fosA; msrE; rmtB; catA1 and mphA. The strains isolated in Yakutsk were classified as ST307 (KL102), ST23 (KL57), ST147 (KL64), and less frequently as ST395 (KL108) and ST3551 (KL64). They carried the genes bla SHV-11/67/106/75/28/182, bla OXA-48, bla OXA-1, bla CTX-M-15, bla TEM-1B, aac (3)-IIa, aac (6›)-Ib-cr, aph (6)- Id, aadA1, rmtF, fosA/6, sul2, catA1/B3, tetA, rmtF. The following genes were identified: efflux pumps oqxAB, KpnFEHG, LptD, msbA, qacE1, H-NS, emrR, CRP, baeR, and marA; porins OmpK37, OmpK36, and OmpA; and the outer membrane vesicle protein MdtQ. Resistance to fluoroquinolones is also caused by mutations in the gyrA (D87N) and parC (S80I) genes. The virulence genes wabG, fimH, and uge_2 were detected in all strains; rmpA was detected in 5 strains belonging to ST395, 23, and 147 from Moscow and Yakutsk; ugeE was detected in 4 strains of the capsular type KL64 (ST395 and 874) from Vologda. Conclusion. Consequently, the resistomes and virulomes of the studied K. pneumoniae strains were characterized, and their affiliation with successful international clones was established.
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