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Wen Shu

Shanghai Jiao Tong University

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AUG 26, 2026 · JOURNAL ARTICLE

Comprehensive evaluation of metagenomic next‐generation sequencing for pathogen identification in patients with suspected infection: Establishment of a standardized validation workflow

Open Research

Rigorous multi-phase validation study with analytical benchmarking, large retrospective cohort comparison, and prospective clinical impact assessment, demonstrating superior sensitivity and turnaround time versus culture; not practice-changing alone because it remains positioned as adjunct to conventional testing.

Strong EvidenceLin Wang Chunyan He Wen Shu

Reported

Qualitative concordance100%

Limit of detection (minimum)10.0 copies/mL

Limit of detection (maximum)269.1 copies/mL

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