SEP 10, 2026 · JOURNAL ARTICLE
In-vitro incubation of human sperm does not change DNA methylation
Human Reproduction Open
A small, single-centre in-vitro study with a null finding; the design is sound but the sample is limited (n=15) and the scope restricted to three media conditions, leaving open whether other culture or earlier developmental stages affect sperm DNA methylation.
Reported
Sample size (donors, methylation…15
Sample size (proteomics analysis)4
Incubation duration24 hours