Methods in Molecular Biology

JOURNAL ARTICLE

Protocol for Detecting and Sequencing Chikungunya Virus from Field-Collected Mosquitoes.

Methods in Molecular Biology

Arboviral diseases represent a major public health challenge, especially in tropical regions where environmental conditions may favor the proliferation and spread of mosquito vecto

JOURNAL ARTICLE

Prime Editing: An Overview.

Methods in Molecular Biology

CRISPR tools are revolutionizing the landscape of genetic therapies, with the potential to cure a range of previously untreatable diseases. Among all the available genome editing t

JOURNAL ARTICLE

Methodological Approaches for the Preservation of Akkermansia muciniphila as Microbial Genetic Resource.

Methods in Molecular Biology

Akkermansia muciniphila is part of the intestinal microbiota and uses mucin as its primary source of carbon and nitrogen. It is considered a promising next-generation probiotic, wi

JOURNAL ARTICLE

Immunophenotyping Studies in Nonhuman Primate Models.

Methods in Molecular Biology

Nonhuman primate (NHP) models are important in biomedical research due to their close evolutionary relationship with humans, which provides a high degree of anatomical, genetic, an

JOURNAL ARTICLE

An Overview of Programmable Epigenetic Editing Based on CRISPR Tools for Gene Expression Regulation.

Methods in Molecular Biology

Epigenetic regulation provides a dynamic and reversible layer of gene control that functions independently of changes in DNA sequence, primarily mediated by DNA methylation, histon

JOURNAL ARTICLE

Base Editing: Mechanisms and Therapeutic Applications.

Methods in Molecular Biology

Base editing enables the direct, programmable conversion of one nucleotide into another at a defined genomic site without introducing a double-strand break. First reported in 2016,

JOURNAL ARTICLE

From Sewers to Surveillance: Monitoring Salmonella Through Wastewater.

Methods in Molecular Biology

Wastewater surveillance (WWS) is a powerful, population-level approach to infectious disease monitoring that complements traditional clinical surveillance, which often under-detect

JOURNAL ARTICLE

Nucleofection-Based CRISPR/Cas Delivery in Human T Cells for Immunotherapy Applications.

Methods in Molecular Biology

Electroporation-based delivery of CRISPR/Cas systems has emerged as a powerful and versatile approach for gene editing in primary human T cells, enabling efficient, transient, and