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Life sciences · Journal article
Methods in Molecular Biology
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Polymerase chain reaction (PCR) is a molecular-based technology that has revolutionized diagnostics and characterization of pathogens, thus affecting how we understand the disease landscape. This technology has been found amenable to application on various strategies for management and control of infectious diseases. The main advantage with PCR technologies, when applied optimally, is the high sensitivity, specificity, efficiency, and short-turn-around time for results, thus rendering the strategy attractive to researchers in infectious diseases and public health. In this chapter, we describe PCR approaches that are innovative and easy to deploy in the detection and characterization of Salmonella, including their antimicrobial resistance (AMR) profiles, in a laboratory with medium-range infrastructure investment.