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Life sciences · Journal article
Revista De Ensino De Bioquímica · October 8, 2026
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INTRODUCTION The BibA protein (Group B Streptococcus Immunogenic Bacterial Adhesin) is an important virulence factor of Streptococcus agalactiae, involved in adhesion to epithelial cells and interaction with the complement regulatory protein C4-binding protein, promoting immune evasion. This surface protein is highly immunogenic and has been investigated as a potential target for vaccine development and diagnostic approaches. In addition to its clinical relevance, BibA can also be used as a didactic model to demonstrate fundamental concepts of microbiology, immunology, and biochemistry in experimental teaching activities. BibA contains an α-helical domain in the N-terminal region, a proline-rich region, and the LPXTG motif at the C-terminal end, responsible for anchoring the protein to the bacterial cell wall. In silico analyses indicate that the BibA fragment (34–622 aa), derived from serotype V and with an approximate molecular weight of 65 kDa, is conserved among major clinical serotypes. OBJECTIVES Previous studies from our research group showed that undergraduate students still present misconceptions about S. agalactiae. In addition, traditional microbiology laboratory practices are not always aligned with current challenges, such as the development of vaccines and new therapeutic strategies. METHODS STRATEGY Considering this context, Real Lab Day (RLD), an inquiry-based teaching initiative associated with the #Adote Project, was developed to bring students closer to scientific practice. The aim of this study was to implement an investigative teaching strategy using BibA as an experimental model and to evaluate student engagement in microbiology research. RESULTS Students participated in laboratory activities involving BibA purification and protein analysis by SDS-PAGE and Western blotting, followed by presentation of their results. Post-activity questionnaires showed that all participants rated their interest in microbiology research as good or excellent, highlighting the potential of this initiative as an active learning strategy. CONCLUSION Real Lab Day strategy effectively integrated theory and practice, increasing student engagement in microbiology research and promoting active, inquiry-based learning.