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Biological Research and Disease Studies / Parasitic Diseases Research and Treatment / Mosquito Borne Diseases and Control · Journal article
Advances in Clinical Medical Research · August 16, 2026
Encouraging direction, but not yet definitive.
This single-centre cross-sectional molecular epidemiology study in Central India identified DENV-2 as the predominant circulating serotype (46.3%) and found it significantly associated with severe dengue (OR 2.91, 95% CI 1.28–6.62; p=0.009). Platelet count <50,000/µL, bleeding manifestations, and abdominal pain were independent predictors of severity. The findings suggest that serotype identification combined with clinical and laboratory markers may improve risk stratification for severe dengue.
Cross-sectional observational study. Clinically suspected dengue patients at a tertiary care hospital in Indore, Madhya Pradesh, India.. Intervention: Molecular serotyping of dengue virus (DENV-1 to DENV-4) by real-time RT-PCR; clinical and hematological assessment.. n = 290. Single centre: tertiary care hospital in Indore, Madhya Pradesh, Central India..
DENV-2 was predominant at 46.3% (31/67), followed by DENV-3 26.9% (18/67), DENV-1 20.9% (14/67), and DENV-4 3.0% (2/67) DENV-2 infection significantly associated with severe dengue: OR 2.91 (95% CI 1.28–6.62; p=0.009) Platelet count <50,000/µL was strongest predictor of severe dengue: OR 4.72 (95% CI 2.01–11.08; p<0.001)
Bleeding manifestations associated with severe dengue: OR 3.18 (p=0.011)
The finding that DENV-2 is significantly associated with severe dengue and that platelet count, bleeding, and abdominal pain predict severity suggests that molecular serotyping and laboratory/clinical assessment may help identify high-risk patients early. However, the observational design and single-centre setting limit generalisability; prospective validation in other populations is needed before changing clinical practice.
A single-centre cross-sectional molecular epidemiology study identifying DENV-2 as predominant and associated with severe dengue (OR 2.91, p=0.009), with clear serotype distribution and clinical correlates, but limited by observational design and modest sample size.
As stated by the source record.
Quoted from the source exactly as published.
The finding that DENV-2 is significantly associated with severe dengue and that platelet count, bleeding, and abdominal pain predict severity suggests that molecular serotyping and laboratory/clinical assessment may help identify high-risk patients early. However, the observational design and single-centre setting limit generalisability; prospective validation in other populations is needed before changing clinical practice.
Graded across the dimensions that decide whether you should act, each from what the source actually supports. There is no single score, and where a dimension was not assessed it says so.
Background: Dengue is an important mosquito-borne viral infection with considerable variation in circulating serotypes and disease severity. Molecular surveillance helps identify predominant serotypes and their association with clinical outcomes. This study evaluated the circulating dengue virus serotypes and their relationship with disease severity in patients from Central India. The aim & objective is to investigate the molecular epidemiology of circulating dengue virus serotypes, identify DENV-1 to DENV-4 by RT-PCR, determine their demographic and clinical distribution, assess their association with disease severity, and generate data to strengthen dengue surveillance and public health interventions. Materials and Methods: A cross-sectional observational study was conducted among 290 clinically suspected dengue patients at a tertiary care hospital in Indore, Madhya Pradesh, from January 2023 to January 2026. Serum samples were screened using the SD BIOLINE™ Dengue Duo Rapid Test. Dengue viral RNA was detected and serotyped using the RealStar® Dengue Type RT-PCR Kit 1.0. Clinical manifestations and hematological parameters were recorded, and multivariate logistic regression was used to identify factors associated with severe dengue. Results: Rapid testing was positive in 61 (21.0%) patients, while real-time RT-PCR confirmed dengue infection in 67 (23.1%). Among RT-PCR-positive cases, DENV-2 was predominant (31, 46.3%), followed by DENV-3 (18, 26.9%), DENV-1 (14, 20.9%), and DENV-4 (2, 3.0%); mixed-serotype infection occurred in 2 (3.0%) cases. DENV-2 infection was significantly associated with severe dengue (OR 2.91, 95% CI: 1.28–6.62; p=0.009). Platelet count <50,000/µL was the strongest predictor (OR 4.72, 95% CI: 2.01–11.08; p<0.001), followed by bleeding manifestations (OR 3.18; p=0.011) and abdominal pain (OR 2.37; p=0.039). Vomiting, abdominal pain, bleeding, and hypotension were significantly associated with serotype. Conclusion: DENV-2 was the predominant circulating serotype and showed a significant association with severe dengue. Molecular serotyping combined with clinical and hematological assessment may improve recognition of patients at risk of severe disease.
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