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Life sciences · Journal article
Amb Express · September 10, 2026
Raises a question worth testing. It does not answer one.
This is a phytochemical isolation and in vitro bioactivity screening study of Stellaria pallida extracts using cell-free assays and computational docking. It identifies several phenolic compounds and reports antioxidant and antimicrobial activity in laboratory assays, but provides no evidence of efficacy in animals or humans and does not validate clinical utility.
Descriptive phytochemical screening with in vitro bioassays and molecular docking analysis. Aerial parts of Stellaria pallida (Caryophyllaceae family plant species); no human or animal subjects.. Intervention: Extraction of S. pallida aerial parts with various organic solvents; chemical characterization and bioactivity screening. Compared with: Multiple solvent extracts compared to each other; no external positive or negative control group reported.
Methanolic extract showed maximum total phenolic content of 58±1.55 mg GAE/g Ethanol extract recorded maximum total flavonoid content of 27.22±0.88 mg QE/g Methanolic extract IC₅₀ values: 16.24 µg/mL (DPPH) and 13.54 µg/mL (H₂O₂ scavenging)
Safety was not reported in the material analysed. Check the source before drawing any conclusion about harm.
The source did not state who this applies to in practice.
This is a mechanistic screening and in silico study of plant phytochemicals with no clinical outcomes, animal models, or in vivo validation; it raises questions about potential antimicrobial activity rather than answering them for clinical use.
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Stellaria pallida L. (Dumort.) is a species that belongs to the Caryophyllaceae family and has a broad range of secondary metabolites and therapeutic potential. In this study, the aerial parts of Stellaria pallida L. ( S. pallida ) were extracted by various organic solvents, viz. Chloroform, ethyl acetate, ethanol, and methanol, among others, were analyzed by GC/MS and HPLC-DAD to determine their chemical composition and antimicrobial properties. In addition, an in silico molecular docking analysis by AutoDock Vina was performed to evaluate the potential interactions between the identified phytochemical compounds and selected antimicrobial target proteins. The methanol extract had the maximum total phenolic (58±1.55 mg GAE/g), and the ethanol extract recorded the maximum total values of flavonoid (27.22±0.88 mg QE/g). Phytol, stigmasterol, Neophytadiene, rutin, chlorogenic acid, ferulic acid, apigenin-7-glucoside, and epicatechin were the major chemical compounds detected in the S. pallida plant. Additionally, the methanolic extract of S. pallida possesses more antioxidant activity in both DPPH and H 2 O 2 scavenging assays, with IC 50 values of 16.24 and 13.54 µg/mL, respectively, compared to other solvent extracts. The antimicrobial evaluation using the agar well diffusion method further confirmed the superior efficacy of the methanolic extract, showing the lowest MIC values against C. albicans (6.25 mg/mL), followed in order by S. aureus and B. subtilis (12.5 mg/mL), while E. coli and K. pneumoniae required higher concentrations (25 mg/mL). Furthermore, molecular docking results revealed strong binding affinities of several identified compounds, particularly rutin and chlorogenic acid, with key antimicrobial target proteins, supporting the observed biological activities. These findings highlight the methanolic extract of S. pallida as a reliable source of natural antioxidants and broad-spectrum antimicrobial activity, in addition to its utility against numerous infectious diseases.
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