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Pasteurella Infections / Swine Diseases / Actinobacillus Infections · Journal article
The Veterinary Quarterly · July 25, 2026
Encouraging direction, but not yet definitive.
This comparative validation demonstrates that micro-bioassay and ELISA correlate highly with LC-MS (Pearson r ≥0.97) for lincomycin quantification in porcine serum and produce comparable pharmacokinetic parameters (Cmax, Tmax, AUC, clearance, mean residence time) in both healthy and co-infected animals. The findings support the use of lower-cost alternatives to LC-MS for generating robust PK/PD data suitable for antimicrobial dose optimization in veterinary settings, particularly in resource-limited environments.
Comparative analytical validation study in porcine pharmacokinetic models. Healthy piglets and piglets co-infected with Actinobacillus pleuropneumoniae and Pasteurella multocida. Intervention: Intramuscular administration of lincomycin at 10 mg/kg BW. Compared with: Three analytical methods: liquid chromatography–mass spectrometry (LC-MS), micro-bioassay (MBa), and enzyme-linked immunosorbent assay (ELISA). Kyungpook National University, South Korea (based on corresponding author affiliation).
Serum concentrations measured by MBa and ELISA showed high correlation with LC-MS in both healthy and infected models (Pearson r ≥0.97) All three methods satisfied standard bioanalytical validation criteria with low limits of detection and quantification, acceptable intra-assay imprecision and error, and strong linearity Disease-associated alterations in selected distribution and disposition parameters observed in co-infected porcine models
Safety was not reported in the material analysed. Check the source before drawing any conclusion about harm.
Veterinary practitioners and researchers in resource-limited settings can employ lower-cost micro-bioassay or ELISA combined with freely available PKSolver software to generate decision-relevant PK/PD data for lincomycin dose optimization in swine, rather than relying exclusively on expensive LC-MS analysis.
A sound analytical validation study comparing three quantification methods in a controlled animal model, demonstrating practical utility for PK/PD analysis but limited to a single antibiotic and veterinary application without clinical outcome data.
As stated by the source record.
Quoted from the source exactly as published.
Veterinary practitioners and researchers in resource-limited settings can employ lower-cost micro-bioassay or ELISA combined with freely available PKSolver software to generate decision-relevant PK/PD data for lincomycin dose optimization in swine, rather than relying exclusively on expensive LC-MS analysis.
Graded across the dimensions that decide whether you should act, each from what the source actually supports. There is no single score, and where a dimension was not assessed it says so.
This study compared liquid chromatography-mass spectrometry (LC-MS), micro-bioassay (MBa), and enzyme-linked immunosorbent assay (ELISA) for quantification of lincomycin (10 mg/kg BW) in porcine serum and evaluated lincomycin pharmacokinetic (PK) data in healthy piglets also co-infected with Actinobacillus pleuropneumoniae and Pasteurella multocida. All methods satisfied standard bioanalytical validation criteria, including low limits of detection and quantification, acceptable intra-assay imprecision and error, and strong linearity across clinically relevant concentration ranges. Serum concentrations measured by MBa and ELISA showed high correlation with LC-MS in both healthy and infected models (Pearson r ≥ 0.97, low bias on Bland-Altman analysis); they produced comparable PK parameters (Cmax, Tmax, area under the curve [AUC], clearance, and mean residence time), indicating suitability for PK/pharmacodynamic (PD)-based dose optimization. PK analysis demonstrated rapid absorption after intramuscular administration and disease-associated alterations in selected distribution and disposition parameters. AUC and clearance were nearly similar across analytical methods and largely consistent between software platforms (WinNonlin and PKSolver). Thus, lower-cost MBa and ELISA-combined with freely available PKSolver-can generate robust, decision-relevant PK/PD data for lincomycin in swine, providing a practical framework for antimicrobial dose refinement and stewardship in resource-limited veterinary and farm settings.
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