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A first descriptive molecular survey of parasite prevalence in a single Portuguese district with limited sample size and no comparative design, providing baseline epidemiological data but requiring larger studies for clinical or public health decision-making.
Retrospective case series from a single tertiary center with modest sample size and no control group; descriptive etiologic and outcome data without comparative statistical analysis or hard clinical endpoints.
A narrative review synthesizing four decades of molecular and epidemiological evidence to conclude that zoonotic Giardia transmission is possible but uncommon, with public health implications for vulnerable populations.
A descriptive seroprevalence survey with a single time point, no comparator, and serological markers only; documents exposure but cannot establish clinical disease, causation, or intervention efficacy.