JUL 26, 2026 · JOURNAL ARTICLE
Systematic Benchmarking of CRISPR-Cas9 Off-Target Prediction Tools Reveals Limitations and Implications for Preclinical Assessment
Human Gene Therapy
A systematic benchmarking study of computational tools against experimental data, showing moderate performance with substantial limitations; informs preclinical practice but does not establish a new clinical standard or definitive tool ranking.
Reported
Sample size (genomic sites)3,827
Number of tools benchmarked14
Guide RNA/Cas9 combinations tested26